Stat3 Polyclonal AntibodyCatalog No DM-Ab-02054Isotype IgGReactivity Human;Mouse;RatApplications IF;WB;IHC;ELISAGene Name STAT3Protein Name Signal transducer and activator of transcription 3Immunogen The antiserum was produced against synthesized peptide derived from humanSTAT3. AA range:672-721Specificity Stat3 Polyclonal Antibody detects endogenous levels of Stat3 protein. Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. Source Polyclonal, Rabbit,IgGPurification The antibody was affinity-purified from rabbit antiserum byaffinity-chromatography using epitope-specific immunogen. Dilution IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Concentration 1 mg/mlPurity ≥90%Storage Stability -20°C/1 yearSynonyms STAT3; APRF; Signal transducer and activator of transcription 3; Acute-phaseresponse factorObserved Band 88kDCell Pathway Cytoplasm . Nucleus . Shuttles between the nucleus and the cytoplasm. Tissue Specificity Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Function disease:Defects in STAT3 are the cause of hyperimmunoglobulin E recurrentELISA: 1/5000. Not yet tested in other applications.Translocated into the nucleus upon tyrosine phosphorylation and dimerization, inresponse to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Expressed in naive CD4(+) T cells as well as T-helper Th17, Th1 and Th2 cells(PubMed:31899195). Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitoryfactor (LIF) stimulation, accumulates in the nucleus. The complex composed ofBART and ARL2 plays an important role in the nuclear translocation and retentioninfection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known ashyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunityand connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctivecoarse facial appearance, abnormal dentition, hyperextensibility of the joints, andbone fractures.,function:Transcription factor that binds to the interleukin-6of STAT3. Identified in a complex with LYN and PAG1.Jiao, Z., Ma, Y., Zhang, Q. et al. The adipose-derivedmesenchymal stem cell secretome promotes hepaticregeneration in miniature pigs after liverischaemia-reperfusion combined with partialresection. Stem Cell Res Ther 12, 218 (2021).Liu, Bowen, et al. "Oncoprotein HBXIP enhancesHOXB13 acetylation and co-activates HOXB13 toconfer tamoxifen resistance in breast cancer." Journalof hematology & oncology 11.1 (2018): 26.Immunofluorescence analysis of Hela cell. 1,Stat3Polyclonal Antibody(red) was diluted at 1:200(4° overnight). β-tubulin Monoclonal Antibody(M7)(green)Liu, Yanmei, et al. "Cancer Stem Cells are Regulatedby STAT3 Signalling in Wilms Tumour." Journal ofCancer 9.8 (2018): 1486.was diluted at 1:200(4° overnight). 2, Goat Anti RabbitAlexa Fluor 594 Catalog:RS3611 was diluted at1:1000(room temperature, 50min). Goat Anti MouseAlexa Fluor 488 Catalog:RS3208 was diluted at1:1000(room temperature, 50min).